Step-One Plus



Real-Time PCR System that offers Fast runs (<40 min) and standard runs (<2 hr). Innovative VeriFlex™ Block for convenient PCR optimization and includes software capable of collecting melt curve data for High Resolution Melt (HRM) applications.


VeriFlex™ Block25°C (5°C zone-to-zone)
Thermal Cycling SystemPeltier-based system
Block Format96-well block
Compatible Consumables• 96-well (0.1 mL) plates with optical flat caps
• 96-well (0.1 mL) plates with optical adhesive cover
• 8-tube (0.1 mL) strips with optical flat caps
• Individual (0.1 mL) tubes with optical flat caps
Supported Volumes10–30 µl
Note: For standard curve experiments, 40 µl in standard mode is validated.
Sample Ramp Rate• Fast mode: +/- 2.2 °C/sec
• Standard mode: +/- 1.6°C/sec
Peak Block Ramp Rate4.6 °C/sec
Temperature Range4°C-100°C
Temperature Accuracy+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start.
Temperature Uniformity+/-0.50°C, 30 seconds after clock start.
Melt Curve ResolutionAs small as 0.1°C
Optical SystemBlue LED excitation source, four-emission filters, and photodiode
Calibrated Dyes at InstallationFAM™, SYBR® Green I, VIC®, JOE™, NED, TAMRA, ROX™ dyes
Passive Reference DyesROX™ or any calibrated dye. Option exists to select no passive reference.
Data CollectionData collected in all 3 filters for all wells regardless of plate setup.
Quantitative PCR Run TimeStandard cycling: Less than 2 hrs
Fast cycling: Less than 40 minutes
  • The StepOnePlus™ System supports many Real-Time PCR applications, including the following:
    • SNP Genotyping
    • Gene Expression Analysis
    • MicroRNA Expression
    • Protein Expression
    • Translocation Analysis
    • Gene Detection
    • Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

  • Standard Curve (absolute quantitation)
  • Relative Standard Curve
  • Comparative Ct (ΔΔCt) (relative quantitation)
  • Genotyping and Presence⁄Absence
  • Melt Curve Analysis (as a standalone application)
  • High Resolution Melting (as a standalone application)


Main Tzabam, building C, room 004


BookIt application


First time users must order a tutoring session.