Super Resolution STED

Leica SPi 8 Gated STED

SR STED
Description

Leica SPi 8 Super-Resolution gSTED Inverted Confocal Microscope

The Leica 3D gSTED system achieves resolution below the diffraction limit in the lateral, and axial directions.

There are 3 gSTED continuous wave lasers at 592 nm (CW), 660 nm (CW) and 775 nm (pulsing laser) which allow the use of fluorophores  From 470 to 670 nm in super-resolution.

Features/Specifications

  • Inverted Leica DMi8 with anti-vibration table
  • Continuous wave laser: UV – Diode 50mW 405nm
  • Pulsed laser: White Light Laser (WLL). Avg. power 1.5mW: 470 – 670nm
  • Depletion lasers: two continuous (CW) wave lasers up to 1.5W at 592 nm, 660 nm and wave pulsing laser at 775 nm 80 MHz (Class 4 lasers)
  • Spectral Detection Range of 400nm – 720nm
  • Detectors: 3 HyD (hybrid detectors) Gated, 2 PMTs, and a TLD (transmitted light detector)
  • Excitation Modulation: up to 8 channels (filters multi-photon ext. & ems.); Acousto Optical Beam Splitter (AOBS): provides selectivity between 0.6 & 2 nm wavelength (no filter set needed).
  • Resonant Scanner 8kHz
  • Accelerated Z-stacking with the SuperZ galvanometer substage
  • 3-D STED (up to 70 nm in Z)
  • HyVolution – SuperResolution for every experiment and every sample (down to 140nm in XY).

Objectives:

  • HC PL FLUOTAR 10x/0.30Plan Fluotar objective, for use with and without coverglass, no immersion.Free working distance: 11.0 mm.
  • HC PL APO 20x/0.75 CS2Plan apochromat objective, no immersion.Free working distance: 0.62 mm.
  • HC PL APO 63x/1.40 OIL CS2Plan apochromat oil immersion objective.Free working distance: 0.14 mm.
  • C PL APO 93X/1.30 GLYC motCORR  STED objective with glycerol immersionMotorized correction collarFree working distance: 0.3 mm.
  • HC PL APO 100x/1.40 Oil STED onlyPlan apochromat oil immersion objective especially designed for STED.Free working distance: 0.13 mm.

Location

Building E, room 006A (SR room)

Reservation

BookIt application

BookIt page

Tutoring

First time users must order a tutoring session.

Site

http://www.leica-microsystems.com