Laser-microdissection

LMD

Laser Microdissection

Leica upright LMD 7

Description

Laser-microdissection (LMD) is a method to isolate cells, single cells, even sub-cellular moieties, under direct microscopic visualization. Thus, LCM can enrich and purify specific targets of interest for downstream applications such as DNA genotyping, RNA-SEQ, cDNA library generation, proteomics, or for cell cloning.

Features/Specifications

  • Upright microscope
  • Cutting-Laser 349 nm , Pulse frequency 10-5000 Hz, Pulse length < 4 ns and Max. pulse energy 120 µJ
  • Camera  Leica DFC7000 T
  • Fully motorized
  • HXP lamp illuminations
LensObjective HCX “PL FLUOTAR” 1.25x/0.04
 Objective UVI “MICRODISSECTION” 5x/0.12 WD 11.7mm
 Objective UVI “MICRODISSECTION” 10x/0.25 WD 11.7mm
 Objective HCX “PL FLUOTAR” 20x/0.4 PH1 WD 6.0 mm
 Objective HCX “PL FLUOTAR” 40x/0.6 PH1
 Objective HCX “PL FLUOTAR” 63x/0.7 PH1
 Objective HCX “PL FLUOTAR MD” 150x/0.9 Dry contact
FiltersFilter BF/PH (empty)
 Filter set LMD-BGR + illumination filter wheel
 Filter set GFP
 Filter set RHOD
 Filter set Y 5

Location

Building E, room 006 (Dark room)

Reservation

BookIt application

BookIt page

Tutoring

First time users must order a tutoring session.

Video tutorial (available only through a direct faculty Ethernet line).

Site

https://www.leica-microsystems.com/products/light-microscopes/p/leica-lmd7/