Laser Microdissection
Leica upright LMD 7
Description
Laser-microdissection (LMD) is a method to isolate cells, single cells, even sub-cellular moieties, under direct microscopic visualization. Thus, LCM can enrich and purify specific targets of interest for downstream applications such as DNA genotyping, RNA-SEQ, cDNA library generation, proteomics, or for cell cloning.
Features/Specifications
- Upright microscope
- Cutting-Laser 349 nm , Pulse frequency 10-5000 Hz, Pulse length < 4 ns and Max. pulse energy 120 µJ
- Camera Leica DFC7000 T
- Fully motorized
- HXP lamp illuminations
| Lens | Objective HCX “PL FLUOTAR” 1.25x/0.04 |
| Objective UVI “MICRODISSECTION” 5x/0.12 WD 11.7mm | |
| Objective UVI “MICRODISSECTION” 10x/0.25 WD 11.7mm | |
| Objective HCX “PL FLUOTAR” 20x/0.4 PH1 WD 6.0 mm | |
| Objective HCX “PL FLUOTAR” 40x/0.6 PH1 | |
| Objective HCX “PL FLUOTAR” 63x/0.7 PH1 | |
| Objective HCX “PL FLUOTAR MD” 150x/0.9 Dry contact | |
| Filters | Filter BF/PH (empty) |
| Filter set LMD-BGR + illumination filter wheel | |
| Filter set GFP | |
| Filter set RHOD | |
| Filter set Y 5 |
Location
Building E, room 006 (Dark room)
Reservation
BookIt application
Tutoring
First time users must order a tutoring session.
Video tutorial (available only through a direct faculty Ethernet line).
Site
https://www.leica-microsystems.com/products/light-microscopes/p/leica-lmd7/